Product Description

Thermo Scientific Dharmacon miRIDIAN® microRNA Mimics and Inhibitors are highly efficient modulators of miRNA activity which help elucidate miRNA involvement in both normal biological processes and disease states. miRIDIAN miRNA Mimics supplement miRNA activity to study gain-of-function effects while miRIDIAN miRNA Inhibitors suppress miRNA activity in loss-of-function experiments.

Benefits

miRIDIAN microRNA Mimics

miRIDIAN microRNA Mimics are double-stranded RNA oligonucleotides, chemically modified with the ON-TARGET® modification pattern for specificity and available for all human, mouse, and rat miRNAs in the Sanger miRBase Sequence Database

• Superior performance in comparison to native-based mimic design
• Effective mimic of endogenous mature miRNA function
• Preferential programming of RISC-like complex with active strand of miRNA and exclusion of passenger strand through proprietary chemical modification patterning

miRIDIAN microRNA Inhibitors

miRIDIAN microRNA Inhibitors are single-stranded chemically-enhanced oligonucleotides available for all human, mouse, and rat miRNAs in the Sanger miRBase Sequence Database

• Effective inhibition of endogenous mature miRNA function by means of innovative design
• Superior potency compared to conventional 21-23mer 2'-O-methyl modified RNA oligonucleotides designed antisense to the mature miRNA
• Persistent inhibition at low concentrations

miRIDIAN microRNA Negative Controls

miRIDIAN microRNA Mimic and Inhibitor Negative Controls are based on C. elegans miRNAs for use as negative experimental controls in human, mouse, and rat cells. The Negative Controls have been analyzed by BLAST against all human, mouse, and rat genomic sequences and miRNA sequences in the miRBase Sequence Database

• Identical design and modifications as miRIDIAN microRNA Mimics and Inhibitors
• No identifiable effects on tested miRNA function in multiple cell lines

miRIDIAN microRNA Libraries

• Complete libraries of miRIDIAN microRNA Mimics and Inhibitors for phenotypic screening applications to study microRNA roles in
  • Stem cell biology
  • Pathological processes
  • Drug treatment outcomes
• Libraries contain microRNA Mimics and Inhibitors for every human, mouse, and rat miRNA in the Sanger miRBase Sequence Database
• Packaged in 96-well plates for convenient high-throughput screening

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