Product Description

The Thermo Scientific Dharmacon® SMARTvector™ shRNA lentiviral platform merges innovations that greatly improve the functionality and specificity of DNA-mediated RNAi and reduce the toxicity associated with low-titer Lentiviral preparations. Dharmacon SMARTvector shRNA products are ready-to-use packaged lentiviral particles that deliver functional and specific shRNA constructs into difficult-to-transfect cells. This includes cells that are resistant to standard transfection such as stem cells, primary cells and neuronal cells

Benefits

SMARTvector shRNA Lentiviral Particles

• Unique microRNA expression scaffold for efficient processing of the shRNA
• Groundbreaking Dharmacon SMARTvector shRNA design algorithm
  • Maximizes selection of functional silencing sequences
  • Seed-based bioinformatic filters and strand bias increase specificity
• Ready-to-use lentiviral particles allow ease of application
  
  • Viral vector system with broad tropism enables delivery into many cell types including diffi cult-to-transfect cells
  • Expression of turboGFP permits visualization of transduction efficiency
  • Generation of stable cell lines facilitated by puromycin selection marker
• Lentigen proprietary manufacturing process yields high-titer (≥108 TU per mL) purified and concentrated lentiviral particle stocks

SMARTvector shRNA Lentiviral Control Particles

Positive Controls

• SMARTvector positive control provided as packaged lentiviral particles
• Consistent, potent silencing of non-essential, abundantly expressed housekeeping genes in human, mouse and rat cells
• Targets Firefly Luciferase expressed from pGL2 and pGL3 (Promega Corp., Madison, WI)

Negative Controls

• SMARTvector negative control provided as packaged lentiviral particles
• Validated to have minimal targeting of any known genes in human, mouse and rat genomes
• Empty vector expressing turboGFP (Evrogen, Moscow, Russia) and puromycin resistance to be used as transduction control

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